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Inhibiting Iron Mobilization from Bacterioferritin in Pseudomonas aeruginosa Impairs Biofilm Formation Irrespective of Environmental Iron Availability
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posted on 2020-01-15, 13:03 authored by Anabel Soldano, Huili Yao, Josephine R. Chandler, Mario RiveraAlthough iron is
essential for bacteria, the nutrient presents problems of toxicity
and solubility. Bacteria circumvent these problems with the aid of
iron storage proteins where Fe3+ is deposited and, when
necessary, mobilized as Fe2+ for metabolic requirements.
In Pseudomonas aeruginosa, Fe3+ is compartmentalized
in bacterioferritin (BfrB), and its mobilization as Fe2+ requires specific binding of a ferredoxin (Bfd) to reduce the stored
Fe3+. Blocking the BfrB-Bfd complex leads to irreversible
iron accumulation in BfrB and cytosolic iron deprivation. Consequently,
given the intracellular iron sufficiency requirement for biofilm development,
we hypothesized that blocking the BfrB-Bfd interaction in P. aeruginosa would impair biofilm development. Our results
show that planktonic and biofilm-embedded cells where the BfrB-Bfd
complex is blocked exhibit cytosolic iron deficiency, and poorly developed
biofilms, even in iron-sufficient culture conditions. These results
underscore inhibition of the BfrB-Bfd complex as a rational target
to dysregulate iron homeostasis and possibly control biofilms.
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dysregulate iron homeostasisbiofilm developmentiron storage proteinsFecytosolic iron deprivationBfrB-BfdPseudomonas aeruginosa Impairs Biofilm Formation IrrespectiveEnvironmental Iron Availabilityiron-sufficient culture conditionsInhibiting Iron Mobilizationexhibit cytosolic iron deficiencyintracellular iron sufficiency requirement
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