mp6b00207_si_001.pdb (7.79 kB)
Indocyanine Green-Loaded Liposomes for Light-Triggered Drug Release
dataset
posted on 2016-04-20, 00:00 authored by Tatu Lajunen, Leena-Stiina Kontturi, Lauri Viitala, Moutusi Manna, Oana Cramariuc, Tomasz Róg, Alex Bunker, Timo Laaksonen, Tapani Viitala, Lasse Murtomäki, Arto UrttiLight-triggered drug
delivery systems enable site-specific and
time-controlled drug release. In previous work, we have achieved this
with liposomes containing gold nanoparticles in the aqueous core.
Gold nanoparticles absorb near-infrared light and release the energy
as heat that increases the permeability of the liposomal bilayer,
thus releasing the contents of the liposome. In this work, we replaced
the gold nanoparticles with the clinically approved imaging agent
indocyanine green (ICG). The ICG liposomes were stable at storage
conditions (4–22 °C) and at body temperature, and fast
near-infrared (IR) light-triggered drug release was achieved with
optimized phospholipid composition and a 1:50 ICG-to-lipid molar ratio.
Encapsulated small molecular calcein and FITC-dextran (up to 20 kDa)
were completely released from the liposomes after light exposure for
15 s. Location of ICG in the PEG layer of the liposomes was simulated
with molecular dynamics. ICG has important benefits as a light-triggering
agent in liposomes: fast content release, improved stability, improved
possibility of liposomal size control, regulatory approval to use
in humans, and the possibility of imaging the in vivo location of
the liposomes based on the fluorescence of ICG. Near-infrared light
used as a triggering mechanism has good tissue penetration and safety.
Thus, ICG liposomes are an attractive option for light-controlled
and efficient delivery of small and large drug molecules.