Indicator
Displacement Assay Inside Dye-Functionalized
Covalent Organic Frameworks for Ultrasensitive Monitoring of Sialic
Acid, an Ovarian Cancer Biomarker
posted on 2020-03-09, 16:06authored byJinmin Wang, Limin Zhao, Bing Yan
Identifying biomolecules
for disease diagnosis requires simple,
accurate, and reliable analytical techniques. Multiple signal transduction
pathways have promoted the development of various biological analysis
systems. However, most systems are largely limited by a single mechanism
or model analysis, which can easily lead to false-positive/negative
results. Herein, we report a covalent organic framework (COF) (TpPa-1) functionalized with a dye (fluorescein sodium) and
design this hybrid material (TpPa-1@Dye) to fabricate
hydrogels for subsequent analysis with the indicator displacement
assay (IDA) method. Selecting a suitable metal cation (Cr3+) for the preparation of hydrogels can reduce the background fluorescence,
improve the detection sensitivity, and increase the corresponding
sensing selectivity. The TpPa-1@Dye functions as an indicator
in the IDA-in-COF system, and Cr3+ is a receptor of the
analyte (sialic acid (SA), a biomarker for ovarian cancer diagnosis).
Based on the above studies, the integrative logic operations (AND
+ IMP) are further established, it helps in elucidating the design
rules of the IDA-in-COF approach. This work represents the first effort
in designing IDA-in-COF luminescent sensors with an On–Off–On
mechanism to determine biomarkers and provides a new approach for
developing hybrid COF luminescent materials as analysis platforms
for human health monitoring.