ac8b01723_si_001.pdf (1.45 MB)
Implementation of Fragment Ion Protection (FIP) during Ultraviolet Photodissociation (UVPD) Mass Spectrometry
journal contribution
posted on 2018-06-21, 00:00 authored by Dustin
D. Holden, James D. Sanders, Chad R. Weisbrod, Christopher Mullen, Jae C. Schwartz, Jennifer S. BrodbeltUltraviolet
photodissociation (UVPD) is a nonselective activation
method in which both precursor and fragment ions may absorb photons
and dissociate. Photoactivation of fragment ions may result in secondary
or multiple generations of dissociation, which decreases the signal-to-noise
ratio (S/N) of larger fragment ions owing to the prevalent subdivision
of the ion current into many smaller, often less informative, fragment
ions. Here we report the use of dipolar excitation waveforms to displace
fragment ions out of the laser beam path, thus alleviating the extent
of secondary dissociation during 193 nm UVPD. This fragment ion protection
(FIP) strategy increases S/N of larger fragment ions and improves
the sequence coverage obtained for proteins via retaining information
deeper into the midsection of protein sequences.