np6b01152_si_001.pdf (2.59 MB)
Identification of Pyridinium with Three Indole Moieties as an Antimicrobial Agent
journal contribution
posted on 2017-03-14, 13:48 authored by Masahiro Okada, Tomotoshi Sugita, Chin Piow Wong, Toshiyuki Wakimoto, Ikuro AbeA novel pyridinium with three indole
moieties, tricepyridinium,
was obtained from the culture of an Escherichia coli clone incorporating metagenomic libraries from the marine sponge Discodermia calyx. For the important structural elements of tricepyridinium to be
investigated for antibacterial activity, tricepyridinium and its analogues
were chemically synthesized. Tricepyridinium had antimicrobial activity,
but not against E. coli, and cytotoxicity against
P388 cells. Additional bioassays with its synthetic analogues revealed
that the intriguing combination of the indole moieties, most likely
derived from three tryptamines, as well as the pyridinium moiety were
chiefly responsible for its potent biological activities.