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Hydrophobic Polyampholytes and Nonfreezing Cold Temperature Stimulate Internalization of Au Nanoparticles to Zwitterionic Liposomes
journal contribution
posted on 2018-06-24, 00:00 authored by Sana Ahmed, Kazuaki Matsumura, Tsutomu HamadaNanomedicine relies on the effective
internalization of nanoparticles
combined with polymeric nanocarriers into living cells. Thus, exploration
of internalization is essential for improving the efficacy of nanoparticle-based
strategies in clinical practice. Here, we investigated the physicochemical
internalization of gold nanoparticles (AuNPs) conjugated with hydrophobic
polyampholytes into cell-sized liposomes at a low but nonfrozen temperature.
The hydrophobic polyampholytes localized in the disordered phase of
the membrane, and internalization of AuNPs was enhanced in the presence
of hydrophobic polyampholytes together with incubation at −3
°C as compared to 25 °C. These results contribute toward
a mechanistic understanding for developing a model nanomaterials-driven
delivery system based on hydrophobic polyampholytes and low temperature.