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Hydrogen Peroxide-Activatable Nanoparticles for Luminescence Imaging and In Situ Triggerable Photodynamic Therapy of Cancer
journal contribution
posted on 2020-03-31, 18:36 authored by Huijie An, Chunhua Guo, Dandan Li, Renfeng Liu, Xiaoqiu Xu, Jiawei Guo, Jun Ding, Jianjun Li, Wei Chen, Jianxiang ZhangAbnormally increased
reactive oxygen species (ROS) are intimately
related to the development and metastasis of cancer. Since hydrogen
peroxide (H2O2) is a major component of ROS,
molecular imaging and selective treatment in response to high H2O2 are intriguing for the management of cancers.
Herein, we report novel self-assembly luminescent nanoparticles, which
can be activated by H2O2, thereby serving as
an effective nanotheranostics for luminescence imaging and in situ photodynamic therapy (PDT) of tumors with high H2O2. This functional nanomedicine was assembled
from an amphiphilic conjugate (defined as CLP) based on chlorin e6
(Ce6) simultaneously conjugated with luminol and poly(ethylene glycol),
exhibiting a well-defined core–shell nanostructure. Upon triggering
by pathologically relevant levels of H2O2, CLP
nanoparticles produced luminescence due to the luminol unit and simultaneous
excitation of Ce6 by chemiluminescence resonance energy transfer,
enabling in vitro and in vivo imaging
of tumors with highly expressed H2O2. In addition,
excited Ce6 can produce singlet oxygen (1O2)
for in situ PDT of H2O2-high
tumors and inhibiting lung metastasis, which was demonstrated by in vitro and in vivo experiments. Furthermore,
preliminary studies revealed the biosafety of CLP nanoparticles. Consequently,
the self-illuminating nanoparticles are promising for noninvasive
imaging and therapy of tumors with high expression of H2O2.