am3022834_si_001.pdf (498.92 kB)
Hydrodynamic Shaping, Polymerization, and Subsequent Modification of Thiol Click Fibers
journal contribution
posted on 2013-01-09, 00:00 authored by Darryl
A. Boyd, Adam R. Shields, Jawad Naciri, Frances S. LiglerHydrodynamic focusing in microfluidic channels is used
to produce highly uniform, shaped polymer fibers at room temperature
and under “green” conditions. Core streams of thiol–ene
and thiol–yne prepolymer solutions were guided using a phase-matched
sheath stream through microfluidic channels with grooved walls to
determine shape. Size was dictated by the ratio of the flow rates
of the core and sheath streams. Thiol click reactions were initiated
using UV illumination to lock in predesigned cross-sectional shapes
and sizes. This approach proved to be much more flexible than electrospinning
in that highly uniform fibers can be produced from prepolymer solutions
with varying compositions and viscosities with made-to-order sizes
and shapes. Furthermore, a very simple manipulation of the composition
provided reactive groups on the fiber surface for attachment of active
ligands and biological components. A proof-of-principle experiment
demonstrated that biotin attached to thiol groups on the fiber surface
could specifically bind a fluorescent protein.