am500883q_si_001.pdf (91.47 kB)
Homogeneous and Sensitive Detection of microRNA with Ligase Chain Reaction and Lambda Exonuclease-Assisted Cationic Conjugated Polymer Biosensing
journal contribution
posted on 2014-05-14, 00:00 authored by Zheng Yuan, Yuanyuan Zhou, Shuxin Gao, Yongqiang Cheng, Zhengping LiA simple and homogeneous microRNA
assay is developed by integration of ligase chain reaction (LCR) and
lambda exonuclease-assisted cationic conjugated polymer (CCP) biosensing.
LCR is utilized for exponential amplification of microRNA, and lambda
exonuclease is introduced to degrade excess fluorescein-labeled probes
in LCR for eliminating background signal. After addition of CCP, efficient
fluorescence resonance energy transfer from CCP to fluorescein in
LCR products occurs. The method is sensitive enough to detect 0.1
fM target microRNA and specific to discriminate one-base difference
of microRNAs, which paves a new way for homogeneous microRNA detection
and molecular diagnosis.