se9b00927_si_001.pdf (1.53 MB)
Highly Sensitive Detection of Bladder Cancer-Related miRNA in Urine Using Time-Gated Luminescent Biochip
journal contribution
posted on 2019-07-30, 20:29 authored by Yingqian Wang, Zhihao Li, Qiaosong Lin, Yurong Wei, Jie Wang, Yingxue Li, Ronghua Yang, Quan YuanDetection
of biomarkers in complex samples is a significant health
plan strategy for medical diagnosis, therapy monitoring, and health
management. However, high background noise resulting from impurities
and other analytes in complex samples has hampered the improvement
of detection sensitivity and accuracy. Herein, an ultralow background
biochip based on time-gated luminescent probes supported by photonic
crystals (PCs) was successfully developed for detection of bladder
cancer (BC)-related miRNA biomarkers with high sensitivity and specificity
in urine samples. Coupled with the time-gated luminescence of long-lifetime
luminescence probes and the luminescence-enhanced capability of PCs,
the short-lived autofluorescence can be efficiently removed; thus,
the detection sensitivity will be significantly improved. Benefiting
from these merits, a detection limit of 26.3 fM is achieved. Furthermore,
the biochip exhibits excellent performance in urinary miRNA detection,
and good recoveries are also obtained. The developed biochip possesses
unique properties of ultralow background and luminescence enhancement,
thus offering a suitable tool for the detection of BC-related miRNA
in urine. With rational design of probe sequences, the biochip holds
great potential for many other biomarkers in real patient samples,
making it valuable in areas such as medical diagnosis and disease
evaluation.