Heterologous Production of 4‑O‑Demethylbarbamide, a Marine Cyanobacterial Natural Product

Kim, Eun Ji; Lee, Jong Hyun; Choi, Hyukjae; Pereira, Alban R.; Ban, Yeon Hee; Yoo, Young Ji; Kim, Eunji; Park, Je Won; Sherman, David H.; Gerwick, William H.; Yoon, Yeo Joon

doi:10.1021/ol302575h.s001

ol302575h_si_001.pdf (1.34 MB)

Heterologous Production of 4‑O‑Demethylbarbamide, a Marine Cyanobacterial Natural Product

journal contribution

posted on 2012-12-07, 00:00 authored by Eun Ji Kim, Jong Hyun Lee, Hyukjae Choi, Alban R. Pereira, Yeon Hee Ban, Young Ji Yoo, Eunji Kim, Je Won Park, David H. Sherman, William H. Gerwick, Yeo Joon Yoon

Heterologous expression of the barbamide biosynthetic gene cluster, obtained from the marine cyanobacterium Moorea producens, in the terrestrial actinobacterium Streptomyces venezuelae, resulted in the production of a new barbamide congener 4-O-demethylbarbamide, demonstrating the potential of this approach for investigating the assembly and tailoring of complex marine natural products.