np7b01063_si_001.pdf (7.81 MB)
HSQC–TOCSY Fingerprinting for Prioritization of Polyketide- and Peptide-Producing Microbial Isolates
journal contribution
posted on 2018-03-02, 20:46 authored by Larissa Buedenbender, Leesa J. Habener, Tanja Grkovic, D. İpek Kurtböke, Sandra Duffy, Vicky M. Avery, Anthony R. CarrollMicrobial products are a promising
source for drug leads as a result
of their unique structural diversity. However, reisolation of already
known natural products significantly hampers the discovery process,
and it is therefore important to incorporate effective microbial isolate
selection and dereplication protocols early in microbial natural product
studies. We have developed a systematic approach for prioritization
of microbial isolates for natural product discovery based on heteronuclear
single-quantum correlation–total correlation spectroscopy (HSQC–TOCSY)
nuclear magnetic resonance profiles in combination with antiplasmodial
activity of extracts. The HSQC–TOCSY experiments allowed for
unfractionated microbial extracts containing polyketide and peptidic
natural products to be rapidly identified. Here, we highlight how
this approach was used to prioritize extracts derived from a library
of 119 ascidian-associated actinomycetes that possess a higher potential
to produce bioactive polyketides and peptides.