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Graphene Oxide/Nucleic-Acid-Stabilized Silver Nanoclusters: Functional Hybrid Materials for Optical Aptamer Sensing and Multiplexed Analysis of Pathogenic DNAs
journal contribution
posted on 2013-08-14, 00:00 authored by Xiaoqing Liu, Fuan Wang, Ruth Aizen, Omer Yehezkeli, Itamar WillnerHybrid
systems consisting of nucleic-acid-functionalized silver nanoclusters
(AgNCs) and graphene oxide (GO) are used for the development of fluorescent
DNA sensors and aptasensors, and for the multiplexed analysis of a series
of genes of infectious pathogens. Two types of nucleic-acid-stabilized
AgNCs are used: one type includes the red-emitting AgNCs (616 nm)
and the second type is near-infrared-emitting AgNCs (775 nm). Whereas
the nucleic-acid-stabilized AgNCs do not bind to GO, the conjugation
of single-stranded nucleic acid to the DNA-stabilized AgNCs
leads to the adsorption of the hybrid nanostructures to GO and to
the fluorescence quenching of the AgNCs. By the conjugation of oligonucleotide
sequences acting as probes for target genes, or as aptamer sequences,
to the nucleic-acid-protected AgNCs, the desorption of the probe/nucleic-acid-stabilized AgNCs from GO through the formation of duplex DNA
structures or aptamer–substrate complexes leads to the generation
of fluorescence as a readout signal for the sensing events. The hybrid
nanostructures are implemented for the analysis of hepatitis B virus
gene (HBV), the immunodeficiency virus gene (HIV), and the syphilis
(Treponema pallidum) gene. Multiplexed analysis of
the genes is demonstrated. The nucleic-acid-AgNCs-modified GO is
also applied to detect ATP or thrombin through the release of the
respective AgNCs-labeled aptamer–substrate complexes from GO.
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oligonucleotide sequencesMultiplexed Analysisimmunodeficiency virus genePathogenic DNAsHybrid systemsduplex DNA structuresfluorescence quenchingreadout signalHBVtarget genesgraphene oxideFunctional Hybrid MaterialsATPhepatitis B virus geneAgNCMultiplexed analysisHIVmultiplexed analysisaptamer sequencesTreponema pallidumOptical AptamerDNA sensors
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