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Granular Sludge Coupling Nitrate/Nitrite Dependent Anaerobic Methane Oxidation with Anammox: from Proof-of-Concept to High Rate Nitrogen Removal
journal contribution
posted on 2019-12-13, 21:45 authored by Sheng-Qiang Fan, Guo-Jun Xie, Yang Lu, Bing-Feng Liu, De-Feng Xing, Hong-Jun Han, Zhiguo Yuan, Nan-Qi RenThis work developed a novel Membrane Granular Sludge Reactor (MGSR) equipped with a gas permeable membrane module for efficient
methane delivery to cultivate nitrate/nitrite dependent anaerobic
methane oxidation (n-DAMO) microorganisms in granular sludge. As proof
of concept, the MGSR was fed with synthetic wastewater containing
nitrate and ammonium to facilitate the growth of n-DAMO microorganisms.
The granular sludge of n-DAMO and Anammox was gradually developed
and achieved a nitrogen removal rate of 1.08 g NO3–-N L–1 d–1 and
0.81 g NH4+-N L–1 d–1. Finally, enriched granular sludge was successfully applied for
nitrogen removal from the synthetic partial nitritation effluent.
The combined dominance of n-DAMO archaea, Anammox bacteria, and n-DAMO
bacteria in the microbial community was confirmed by 16S rRNA amplicon
sequencing. Fluorescence in situ hybridization revealed that a layered
structure was formed in the granular sludge with Anammox bacteria
in the outer layer and n-DAMO microorganisms in the inner layer when
granules were fed with nitrite and ammonium. The high performance
of nitrogen removal (16.53 kg N m–3 d–1) with satisfactory effluent quality (∼8 mg N L–1) and excellent biomass retention capacity (43 g VSS L–1) make the MGSR promising for the practical application of n-DAMO
and Anammox in wastewater treatment.