jp065121l_si_001.pdf (18.04 kB)
Gold Nanoparticle Based FRET Asssay for the Detection of DNA Cleavage
journal contribution
posted on 2006-10-26, 00:00 authored by Paresh Chandra Ray, Angela Fortner, Gopala Krishna DarbhaWe report gold nanoparticle based FRET assay to monitor the cleavage of DNA by nucleases. Fluorescence
signal enhancement is observed by a factor of 120 after the cleavage reaction in the presence of S1 nuclease.
The mechanism of distant dependent fluorescence quenching has been discussed. Our experimental results
on distance dependent fluorescence quenching match quite well with theoretical findings obtained from the
fluorescence quenching model by Gersten and Nitzan (Surf. Sci. 1985, 158, 165). Our experimental observation
paradigm for the design of optical based molecular ruler strategies at distances more than double the distances
achievable using traditional dipole−dipole Columbic energy transfer based methods.