am0c01167_si_001.pdf (1.86 MB)
Gemini Peptide Amphiphiles with Broad-Spectrum Antimicrobial Activity and Potent Antibiofilm Capacity
journal contribution
posted on 2020-03-31, 17:27 authored by Ruilian Qi, Na Zhang, Pengbo Zhang, Hao Zhao, Jian Liu, Jie Cui, Junfeng Xiang, Yuchun Han, Shu Wang, Yilin WangTo
address the challenge from microbial resistance and biofilm,
this work develops three gemini peptide amphiphiles with basic tetrapeptide
spacers 12-(Arg)4-12, 12-(Lys)4-12, and 12-(His)4-12 and finds that they exhibit varied antimicrobial/antibiofilm
activities. 12-(Arg)4-12 shows the best performance, possessing
the broad-spectrum antimicrobial activity and excellent antibiofilm
capacity. The antimicrobial and antibiofilm activities strongly depend
on the membrane permeation and self-assembling structure of these
peptide amphiphiles. Gemini peptide amphiphile with highly polar arginine
as the spacer, 12-(Arg)4-12, self-assembles into short
rods that are prone to dissociate into monomers for permeating and
lysing membrane , leading to its broad-spectrum antimicrobial activity
and high efficiency in eradicating biofilm. Long rods formed by relatively
weaker polar 12-(Lys)4-12 are less prone to disassemble
into monomers for further membrane permeation, which makes it selectively
kill more negatively charged bacteria and endow it medium antibiofilm
activity. Low polar 12-(His)4-12 aggregates into long fibers,
which are very difficult to dissociate and they mainly electrostatically
bind on the negative microbial surface, resulting in its weakest antimicrobial
and antibiofilm activity. This study reveals the effect of the antimicrobial
peptide structure and aggregation on the antimicrobial activities
and would be helpful for developing high-efficient antimicrobial peptides
with antibiofilm activity.
History
Usage metrics
Categories
Keywords
broad-spectrum antimicrobial activitymedium antibiofilm activity4 -124 -12 aggregatesgemini peptide amphiphilesantimicrobial peptide structureGemini Peptide Amphiphilesantibiofilm activityGemini peptide amphiphileBroad-Spectrum Antimicrobial ActivityPotent Antibiofilm Capacityhigh-efficient antimicrobial peptidesmembrane permeationArg
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC