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Functional Biodegradable Nitric Oxide Donor-Containing Polycarbonate-Based Micelles for Reduction-Triggered Drug Release and Overcoming Multidrug Resistance
journal contribution
posted on 2019-11-14, 13:09 authored by Leilei Gao, Bin Dong, Junmei Zhang, Ying Chen, Haishi Qiao, Zhihong Liu, Enping Chen, Yuqin Dong, Chongjiang Cao, Dechun Huang, Wei ChenNitric oxide (NO), as a bioeffector to improve chemosensitivity
by reversing multidrug resistance (MDR), is highly attractive for
developing combinational delivery systems to deal with MDR tumors,
while it is highly challenged by the stability and controlled release
of NO during the pathway. Here we design and synthesize a cyclic nitrate
trimethylene carbonate monomer (NTC), followed by ring-opening polymerization
to prepare amphiphilic biodegradable polycarbonate-based copolymers
as polymeric NO donors with tailored contents. The copolymer with
desirable molecular weight is readily self-assembled to biodegradable
micelles (NO-M) with a uniform size of 130 nm for highly stabilizing
NO donors at the physiological conditions, while triggered NO release
from micelles is performed at the intracellular reduction conditions.
More importantly, NO-M shows superior inhibition of P-gP expression
to enhance the chemosensitivity of multidrug-resistant MCF7 cells
(MCF7/DOXR). DOX-loaded NO-M (NO-M@DOX) realizes fast DOX
release at the intracellular conditions, resulting in more intracellular
DOX accumulation and higher antitumor activity mediated by the reduction-triggered
NO/DOX release and NO-induced MDR reversal. Furthermore, the in vivo
results show that NO-M@DOX effectively suppresses the MCF7/DOXR tumor growth by a combination of directly NO-induced therapy
and NO-mediated enhanced chemotherapy; meanwhile, the treatment with
NO-M systems have much fewer side effects.
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Keywords
Reduction-Triggered Drug Releasepolycarbonate-based copolymersside effectsintracellular conditionsring-opening polymerizationNitric Oxide Donor-Containing Polycarbonate-Based Micelles130 nmintracellular DOX accumulationcombinational delivery systemsDOX-loaded NO-Muniform sizeDOX releasecyclic nitrate trimethylene carbonate monomermultidrug-resistant MCF 7 cellsP-gP expressionmultidrug resistanceNO-induced therapyNTCNO-M systemsintracellular reduction conditionsOvercoming Multidrug Resistance Nitric oxidevivo results showantitumor activityNO-induced MDR reversalMDR tumors
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