nl9b01732_si_liveslides.zip (6.34 MB)
Force-Dependent Regulation of Talin–KANK1 Complex at Focal Adhesions
online resource
posted on 2019-10-18, 20:29 authored by Miao Yu, Shimin Le, York-Christoph Ammon, Benjamin T. Goult, Anna Akhmanova, Jie YanKANK
proteins mediate cross-talk between dynamic microtubules and
integrin-based adhesions to the extracellular matrix. KANKs interact
with the integrin/actin-binding protein talin and with several components
of microtubule-stabilizing cortical complexes. Because of actomyosin
contractility, the talin–KANK complex is likely under mechanical
force, and its mechanical stability is expected to be a critical determinant
of KANK recruitment to focal adhesions. Here, we quantified the lifetime
of the complex of the talin rod domain R7 and the KN domain of KANK1
under shear-force geometry and found that it can withstand forces
for seconds to minutes over a physiological force range up to 10 pN.
Complex stability measurements combined with cell biological experiments
suggest that shear-force stretching promotes KANK1 localization to
the periphery of focal adhesions. These results indicate that the
talin–KANK1 complex is mechanically strong, enabling it to
support the cross-talk between microtubule and actin cytoskeleton
at focal adhesions.