ac5020782_si_001.pdf (8.58 MB)
Folding Analytical Devices for Electrochemical ELISA in Hydrophobic RH Paper
journal contribution
posted on 2014-12-16, 00:00 authored by Ana C. Glavan, Dionysios
C. Christodouleas, Bobak Mosadegh, Hai Dong Yu, Barbara S. Smith, Joshua Lessing, M. Teresa Fernández-Abedul, George M. WhitesidesThis
work describes a device for electrochemical enzyme-linked
immunosorbent assay (ELISA) designed for low-resource settings and
diagnostics at the point of care. The device is fabricated entirely
in hydrophobic paper, produced by silanization of paper with decyl
trichlorosilane, and comprises two zones separated by a central crease:
an embossed microwell, on the surface of which the antigen or antibody
immobilization and recognition events occur, and a detection zone
where the electrodes are printed. The two zones are brought in contact
by folding the device along this central crease; the analytical signal
is recorded from the folded configuration. Two proof-of-concept applications,
an electrochemical direct ELISA for the detection of rabbit IgG as
a model antigen in buffer and an electrochemical sandwich ELISA for
the detection of malarial histidine-rich protein from Plasmodium falciparum (Pf HRP2)
in spiked human serum, show the versatility of this device. The limit
of detection of the electrochemical sandwich ELISA for the quantification
of Pf HRP2 in spiked human serum was 4 ng mL–1 (102 pmol L–1), a value
within the range of clinically relevant concentrations.