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Flexible Three-Dimensional Net for Intravascular Fishing of Circulating Tumor Cells
journal contribution
posted on 2020-03-20, 11:05 authored by Shi-Bo Cheng, Ming Wang, Chi Zhang, Miao-Miao Chen, Yi-Ke Wang, Shan Tian, Na Zhan, Wei-Guo Dong, Min Xie, Wei-Hua HuangCurrent strategies
for in vitro isolation of circulating
tumor cells (CTCs) fail to detect extremely rare CTCs heterogeneously
distributed in blood. It is possible to devise methods for in vivo capture of CTCs based on processing almost all of
the blood in the human body to improve detection sensitivity, but
the complicated manipulation, biosafety concerns, and limited capture
efficiency of conventional detection strategies prohibit their implementation
in the clinic. Herein, we present a flexible three-dimensional (3-D)
CTC-Net probe for intravascular collection of CTCs. The CTC-Net, consisting
of a 3-D elastic scaffold with an interconnected, spatially distributed
network accommodates a large quantity of immobilized antibodies and
provides an enhanced substrate–cell contact frequency, which
results in an enhanced capture efficiency and effective detection
of heterogeneous CTCs. The as-prepared CTC-Net can be readily compressed
and injected into blood vessels and fully unfolded to form a 3-D “fishing-net”
structure for capture of the CTCs, and then retracted for imaging
and downstream gene analysis of the captured CTCs. Significant advantages
for the CTC-Net over currently available in vitro and in vivo procedures are demonstrated for detection
of extremely rare CTCs from wild-type rats and successful capture
of CTCs and CTC clusters before metastasis in the case of tumor-bearing
rats. Our research demonstrates for the first time the use of a 3-D
scaffold CTC-Net probe for in vivo capture of CTCs.
The method shows exceptional performance for cell capture, which is
readily implemented and holds great potential in the clinic for early
diagnosis of cancer.
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as-prepared CTC-Netbiosafety concernsdetection sensitivitywild-type ratsSignificant advantages3- Dintravascular collectiongene analysistumor cells3- D scaffold CTC-Net probevivo proceduresCirculating Tumor Cellsblood vesselsCTC-Net probeIntravascular FishingCTC clusterstumor-bearing ratsFlexible Three-Dimensionaldetection strategiesCTCs heterogeneously
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