bc9b00561_si_001.pdf (255.04 kB)
Flavin Binding Allosteric Aptamer with Noncovalent Labeling for miR Sensing
Version 2 2019-10-11, 19:16
Version 1 2019-10-09, 19:34
journal contribution
posted on 2019-10-11, 19:16 authored by A. Gee, J. A. Grennell, S. Sitaula, J. Jayawickramarajah, M. F. AliModular allosteric aptamers with
discrete recognition and signaling
regions provide a facile method of carrying out label-free detection
by forgoing complex target labeling requirements. Herein, we describe
the design and function of an aptamer scaffold capable of forming
a hairpin loop in the presence of FAD (the signaling trigger). The
aptamer includes a recognition region for the microRNA (miR) Let-7i.
Upon selective miR hybridization, the aptamer undergoes a conformational
shift to release FAD and thus produce a measurable response. As a
result, the described method can sensitively and selectively detect
miR Let-7i with a wide linear range of 0.1 pM to 1 μM and a
detection limit of 150 fM. Additionally, this strategy was able to
selectively discriminate between sequences with 1- and 2-nucleotide
(nt) differences.