ph0c00027_si_001.pdf (278.54 kB)
Fighting against Fast Speckle Decorrelation for Light Focusing inside Live Tissue by Photon Frequency Shifting
journal contribution
posted on 2020-03-09, 17:03 authored by Jiamiao Yang, Lei Li, Jingwei Li, Zhongtao Cheng, Yan Liu, Lihong V. WangLight
focusing inside live tissue by digital optical phase conjugation
(DOPC) has drawn increasing interest due to its potential biomedical
applications in optogenetics, microsurgery, phototherapy, and deep-tissue
imaging. However, fast physiological motions in a live animal, including
blood flow and respiratory motions, produce undesired photon perturbation
and thus inevitably deteriorate the performance of light focusing.
Here, we develop a photon-frequency-shifting DOPC method to fight
against fast physiological motions by switching the states of a guide
star at a distinctive frequency. Therefore, the photons tagged by
the guide star are well detected at the specific frequency, separating
them from the photons perturbed by fast motions. Light focusing was
demonstrated in both phantoms in vitro and mice in vivo with substantially
improved focusing contrast. This work puts a new perspective on light
focusing inside live tissue and promises wide biomedical applications.