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Fast and Online Determination of Five Avermectin Residues in Foodstuffs of Plant and Animal Origin Using Reusable Polymeric Monolithic Extractor Coupled with LC-MS/MS
journal contribution
posted on 2015-04-29, 00:00 authored by Xin Li, Man-Man Wang, Guo-Ying Zheng, Lian-Feng Ai, Xue-Sheng WangA hydrophobic monolith (10 mm ×
2.1 mm i.d.) was developed as a reusable online solid-phase extraction
(SPE) sorbent coupled with LC-MS/MS for the rapid determination of
five avermectin residues in foodstuffs of both plant and animal origin.
The online SPE was achieved using a 10 mmol/L ammonium acetate solution
as the loading solvent, and acetonitrile (MeCN) was selected for the
washing step. After being transferred from the monolith into a C18 analytical column using MeCN, the analytes were analyzed
by LC-MS/MS using MeCN/0.1% NH4OH (10:90, v/v) as the mobile
phase. The detection limit was 2 μg/kg for five avermectins,
and the recoveries in fresh pear, chili seed, bovine muscle, and milk
ranged from 71.8% to 101.3% with relative standard deviations of less
than 8.94%. The online SPE and determination were achieved within
15 min, and the monolithic extractor was reusable for more than 500
experiments.