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Fast Online Separation and Identification of Electrochemically Generated Isomeric Oxidation Products by Trapped Ion Mobility–Mass Spectrometry
journal contribution
posted on 2019-12-13, 15:36 authored by Jens Fangmeyer, Simon G. Scheeren, Robin Schmid, Uwe KarstTrapped ion mobility spectrometry (TIMS) is presented
as a new
and rapid method to distinguish between electrochemically generated
isomeric oxidation products. Separation was performed online directly
after generation and ionization of the analytes, thus providing the
opportunity to detect even short-lived and reactive transformation
products. The same setup enables structure elucidation based on TIMS
aligned fragmentation experiments. Due to the high resolution, TIMS
was able to distinguish between two isomeric transformation products
of the model compound metoprolol, which only differ in the position
of the hydroxylation taking place in the benzylic and aromatic positions,
respectively. Using this method, the analysis time is at least five
times shorter compared to conventional chromatography approaches.
Consequently, TIMS may arise as a powerful tool in electrochemical
metabolism studies.
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Electrochemically Generated Isomeric Oxidation Productsisomeric transformation productsreactive transformation productsion mobility spectrometryelectrochemical metabolism studiesmethodFast Online Separationisomeric oxidation productsTIMSmodel compound metoprololstructure elucidationfragmentation experimentsanalysis time
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