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Facile Synthesis of Magnetic Mesoporous Hollow Carbon Microspheres for Rapid Capture of Low-Concentration Peptides
journal contribution
posted on 2015-12-17, 03:42 authored by Gong Cheng, Ming-Da Zhou, Si-Yang ZhengMesoporous
and hollow carbon microspheres embedded with magnetic nanoparticles
(denoted as MHM) were prepared via a facile self-sacrificial method
for rapid capture of low-abundant peptides from complex biological
samples. The morphology, structure, surface property, and magnetism
were well-characterized. The hollow magnetic carbon microspheres have
a saturation magnetization value of 130.2 emu g–1 at room temperature and a Brunauer–Emmett–Teller specific
surface area of 48.8 m2 g–1 with an average
pore size of 9.2 nm for the mesoporous carbon shell. The effectiveness
of these MHM affinity microspheres for capture of low-concentration
peptides was evaluated by standard peptides, complex protein digests,
and real biological samples. These multifunctional hollow carbon microspheres
can realize rapid capture and convenient separation of low-concentration
peptides. They were validated to have better performance than magnetic
mesoporous silica and commercial peptide-enrichment products. In addition,
they can be easily recycled and present excellent reusability. Therefore,
it is expected that this work may provide a promising tool for high-throughput
discovery of peptide biomarkers from biological samples for disease
diagnosis and other biomedical applications.