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Extraction, Enrichment, and in situ Electrochemical Detection on Lab-on-a-Disc: Monitoring the Production of a Bacterial Secondary Metabolite
journal contribution
posted on 2018-12-11, 00:00 authored by Sune Zoëga Andreasen, Kuldeep Sanger, Christian Bille Jendresen, Alex Toftgaard Nielsen, Jenny Emnéus, Anja Boisen, Kinga ZórDevelopment
of microsystems, which enable “sample-to-answer”
detection from real samples, is often challenging. We present the
first integration of supported liquid membrane extraction combined
with electrochemical detection on a centrifugal fluidic platform.
The developed lab-on-a-disc (LoD) system enabled the separation, enrichment,
and subsequent electrochemical detection of the target analyte from
a complex sample mixture. As a case study, we quantified the amount
of a dietary supplement and pharmaceutical precursor, p-coumaric acid,
from bacterial growth media at different time points during production.
The assay, extraction, and detection, performed on the LoD device,
proved to be a low cost and environmentally friendly approach, requiring
only a few tens of microliters of organic solvent and enabled detection
in a 3 μL volume. In addition, the data obtained from the centrifugal
platform showed a good correlation with data obtained from the high
performance liquid chromatography analysis.