ao8b01713_si_003.xlsx (68.29 kB)
Exploring Exercise- and Context-Induced Peptide Changes in Mice by Quantitative Mass Spectrometry
dataset
posted on 2018-10-22, 14:37 authored by Sarah
E. Dowd, Martina L. Mustroph, Elena V. Romanova, Bruce R. Southey, Heinrich Pinardo, Justin S. Rhodes, Jonathan V. SweedlerRecent research suggests that exercise
may help facilitate abstinence
from cocaine addiction, though the mechanisms are not well understood.
In mice, wheel running accelerates the extinction of conditioned place
preference (CPP) for cocaine, providing an animal model for evaluating
potential neurological mechanisms. The objective of this study was
to quantify dynamic changes in endogenous peptides in the amygdala
and dentate gyrus of the hippocampus in mice exposed to a context
paired with the effects of cocaine, and in response to exercise. Male
C57BL/6J mice conditioned to cocaine were housed with or without running
wheels for 30 days. Following a CPP test and final exposure to either
a cocaine- or saline-associated context, peptides were measured in
brain tissue extracts using label-free matrix-assisted laser desorption/ionization
mass spectrometry (MS) and stable isotopic labeling with liquid chromatography
and electrospray ionization MS. CPP in mice was significantly reduced
with running, which correlated to decreased myelin basic protein derivatives
in the dentate gyrus extracts, possibly reflecting increased unmyelinated
granule neuron density. Exposure to a cocaine-paired context increased
hemoglobin-derived peptides in runners and decreased an actin-derived
peptide in sedentary animals. These results allowed us to characterize
a novel set of biomarkers that are responsive to exercise in the hippocampus
and in a cocaine-paired context in the amygdala.