jp5b01432_si_001.pdf (1.88 MB)
Excited State Dynamics of the Isolated Green Fluorescent Protein Chromophore Anion Following UV Excitation
journal contribution
posted on 2015-03-12, 00:00 authored by Christopher
W. West, James N. Bull, Alex S. Hudson, Steven L. Cobb, Jan R. R. VerletA combined frequency-, angle-, and
time-resolved photoelectron
spectroscopy study is used to unravel the excited state dynamics following
UV excitation of the isolated anionic chromophore of the green fluorescent
protein (GFP). The optically bright S3 state, which is
populated for hv > 3.7 eV, is shown to decay predominantly
by internal conversion to the S2 state that in turn autodetaches
to the neutral ground state. For hv > 4.1 eV,
a new
and favorable autodetachment channel from the S2 state
becomes available, which leads to the formation of the neutral in
an excited state. The results indicate that the UV excited state dynamics
of the GFP chromophore involve a number of strongly coupled excited
states.