pr6b00471_si_002.xlsx (10.14 kB)
Examination of Endogenous Peptides in Medicago truncatula Using Mass Spectrometry Imaging
dataset
posted on 2016-09-22, 00:00 authored by Erin Gemperline, Caitlin Keller, Dhileepkumar Jayaraman, Junko Maeda, Michael
R. Sussman, Jean-Michel Ané, Lingjun LiPlant
science is an important, rapidly developing area of study.
Within plant science, one area of study that has grown tremendously
with recent technological advances, such as mass spectrometry, is
the field of plant-omics; however, plant peptidomics is relatively
underdeveloped in comparison with proteomics and metabolomics. Endogenous
plant peptides can act as signaling molecules and have been shown
to affect cell division, development, nodulation, reproduction, symbiotic
associations, and defense reactions. There is a growing need to uncover
the role of endogenous peptides on a molecular level. Mass spectrometric
imaging (MSI) is a valuable tool for biological analyses as it allows
for the detection of thousands of analytes in a single experiment
and also displays spatial information for the detected analytes. Despite
the prediction of a large number of plant peptides, their detection
and imaging with spatial localization and chemical specificity is
currently lacking. Here we analyzed the endogenous peptides and proteins
in Medicago truncatula using matrix-assisted laser
desorption/ionization (MALDI)–MSI. Hundreds of endogenous peptides
and protein fragments were imaged, with interesting peptide spatial
distribution changes observed between plants in different developmental
stages.