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Evidence of an Unusual Poly(A) RNA Signature Detected by High-Throughput Chemical Mapping
journal contribution
posted on 2020-05-21, 23:13 authored by Roger Wellington-Oguri, Eli Fisker, Mathew Zada, Michelle Wiley, Jill Townley, Eterna PlayersHomopolymeric
adenosine RNA plays numerous roles in both cells
and noncellular genetic material. We report herein an unusual poly(A)
signature in chemical mapping data generated by the Eterna Massive
Open Laboratory. Poly(A) sequences of length seven or more show unexpected
results in the selective 2′-hydroxyl acylation read out by
primer extension (SHAPE) and dimethyl sulfate (DMS) chemical probing.
This unusual signature first appears in poly(A) sequences of length
seven and grows to its maximum strength at length ∼10. In a
long poly(A) sequence, the substitution of a single A by any other
nucleotide disrupts the signature, but only for the 6 or so nucleotides
on the 5′ side of the substitution.