American Chemical Society
Browse
bi0c00215_si_001.pdf (692.68 kB)

Evidence of an Unusual Poly(A) RNA Signature Detected by High-Throughput Chemical Mapping

Download (692.68 kB)
journal contribution
posted on 2020-05-21, 23:13 authored by Roger Wellington-Oguri, Eli Fisker, Mathew Zada, Michelle Wiley, Jill Townley, Eterna Players
Homopolymeric adenosine RNA plays numerous roles in both cells and noncellular genetic material. We report herein an unusual poly­(A) signature in chemical mapping data generated by the Eterna Massive Open Laboratory. Poly­(A) sequences of length seven or more show unexpected results in the selective 2′-hydroxyl acylation read out by primer extension (SHAPE) and dimethyl sulfate (DMS) chemical probing. This unusual signature first appears in poly­(A) sequences of length seven and grows to its maximum strength at length ∼10. In a long poly­(A) sequence, the substitution of a single A by any other nucleotide disrupts the signature, but only for the 6 or so nucleotides on the 5′ side of the substitution.

History