la300325p_si_001.pdf (9.07 MB)
Evidence for Patchy Lipid Layers on Gold Nanoparticle Surfaces
journal contribution
posted on 2016-02-21, 16:30 authored by Jie An Yang, Catherine J. MurphyGold nanoparticles bearing multiple surface ligands are
becoming
favored candidates as multifunctional targeting, imaging, and therapeutic
vehicles for biomedicine. The question of spatial location of different
ligands on nanoparticle surfaces, especially with those of diameters
less than 100 nm, is an important one that is difficult to quantitatively
address. Here we functionalize the surface of 20, 50, and 90 nm gold
nanoparticles with two different lipids, both single and mixed, using
two different surface chemical procedures. Mass spectrometry supports
the presence of both lipids in the mixed-lipid systems on nanoparticles,
while electron microscopy evidence shows domain sizes for one lipid
apparently a quarter to a half the projected diameter for 50 and 90
nm particles; but for 20 nm particles, there is no evidence for the
existence of patches of the two lipids. Larger gold nanoparticles
(90 nm) can be decorated with an array of 12 nm gold nanoparticles
by use of a third lipid and antibody–antigen connectors; the
display of the 12 nm particles about the 90 nm particles can be controlled
to some extent by the initial surface chemistry and is quantified
via a new angle analysis procedure.
History
Usage metrics
Categories
Keywords
nanoparticle surfaceselectron microscopy evidencemass spectrometryPatchy Lipid Layerssurface chemical procedures12 nm particlessurface chemistrydomain sizes12 nm gold nanoparticles90 nm gold nanoparticleslipid20 nm particlesangle analysis procedure100 nm90 nm particlesGold Nanoparticle SurfacesGold nanoparticlessurface ligands
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC