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Epitope Molecularly Imprinted Polymer Nanoparticles for Chemo-/Photodynamic Synergistic Cancer Therapy Guided by Targeted Fluorescence Imaging
journal contribution
posted on 2020-03-05, 19:11 authored by Hui Peng, Ya-Ting Qin, Xi-Wen He, Wen-You Li, Yu-Kui ZhangIt
is a still tough task to precisely target cancer cells and efficiently
improve the therapeutic efficacy of various therapies at the same
time. Here, dual-template imprinting polymer nanoparticles (MIPs)
with a core–shell structure were prepared, in which fluorescent
silica nanoparticles (FSiO2) were the core and the imprinted
polymer layers were the outermost shell. The imprinted layer was designed
and constructed via free-radical precipitation approach on the surface
of FSiO2, which simultaneously encapsulated gadolinium-doped
silicon quantum dots and photosensitizers (Ce6). During the polymerization
process, two template molecules were introduced into the mixtures,
one was the epitope of CD59 protein (YNCPNPTADCK), which was overexpressed
on the surface of a great deal of the solid cancers, and the other
was antitumor agent doxorubicin (DOX) to be used for chemotherapy.
Furthermore, the embedded Ce6 could generate toxic 1O2 under 655 nm laser irradiation to kill cancer cells, combining
with the loaded-DOX to obtain a synergistic cancer therapy. Moreover,
owing to the introduction of gadolinium-doped silicon quantum dots,
Ce6, and DOX, the MIPs were endowed with targeted fluorescence imaging
(FI) and MR imaging (MRI). In vitro and in vivo experiments had been
conducted to demonstrate the excellent targeting ability and desirable
treatment effect with negligible toxicity to healthy tissues and organs.
As a consequence, the designed MIPs can promote the development of
targeted recognition against biomarkers and precise treatment guided
with cell imaging tools.
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free-radical precipitation approachEpitope Molecularly Imprinted Polymer Nanoparticlesgadolinium-doped silicon quantum dotstarget cancer cellstherapyTargeted Fluorescence ImagingCD 59 proteincell imaging toolsMIPscoreMRencapsulated gadolinium-doped silicon quantum dotsdual-template imprinting polymer nanoparticlesDOXFIFSiO 21 O 2CeYNCPNPTADCKsurfaceantitumor agent doxorubicin655 nm laser irradiationMRI
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