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Enhancing Mucosal Immune Response of Newcastle Disease Virus DNA Vaccine Using N‑2-Hydroxypropyl Trimethylammonium Chloride Chitosan and N,O‑Carboxymethyl Chitosan Nanoparticles as Delivery Carrier
Version 2 2017-12-07, 18:07
Version 1 2017-12-06, 16:12
journal contribution
posted on 2017-11-27, 00:00 authored by Kai Zhao, Jinyu Han, Yang Zhang, Lin Wei, Shuang Yu, Xiaohua Wang, Zheng Jin, Yunfeng WangBecause mucosal sites are the entry
ports of pathogens, immunization via mucosal routes
can extremely enhance the immunity. To
elevate the potential of N-2-hydroxypropyl trimethylammonium
chloride chitosan (N-2-HACC) and N,O-carboxymethyl chitosan (CMC) nanoparticles as a mucosal immune delivery
carrier for DNA vaccines, we prepared the NDV F gene plasmid DNA with
C3d6 molecular adjuvant (pVAX I-F(o)-C3d6) encapsulated in the N-2-HACC-CMC
nanoparticles (N-2-HACC-CMC/pFDNA-C3d6 NPs). The N-2-HACC-CMC/pFDNA-C3d6
NPs had regular spherical morphology and low toxicity with a mean
diameter of 309.7 ± 6.52 nm, zeta potential of 49.9 ± 4.93
mV, encapsulation efficiency of 92.27 ± 1.48%, and loading capacity
of 50.75 ± 1.35%. The N-2-HACC-CMC had high stability and safety.
The pVAX I-F(o)-C3d6 could be sustainably released from the N-2-HACC-CMC/pFDNA-C3d6
NPs after an initial burst release. Immunization intranasally of chickens
with N-2-HACC-CMC/pFDNA-C3d6 NPs not only produced higher anti-NDV
IgG and sIgA antibody than chickens in other groups did, but also
significantly stimulated lymphocyte proliferation and triggered higher
the IL-2, IL-4, and IFN-γ levels. These findings indicated that
the N-2-HACC-CMC could be used as an efficient delivery carrier for
the mucosal immunity of Newcastle disease virus DNA vaccine. The work
laid a basis for the quaternized chitosan nanoparticles as efficient
mucosal immunity delivery carrier for DNA vaccines and had immense
application promise and potential for vaccines and drugs.
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quaternized chitosan nanoparticlesIFN -γ levelsdelivery carrierDNA vaccinesCMCNewcastle Disease Virus DNA VaccineNewcastle disease virus DNA vaccineI-FN -2- hydroxypropyl trimethylammonium chloride chitosanEnhancing Mucosal Immune Responsemucosal immunity delivery carrierN -2-HACC nanoparticlesN -2-HACCILN -2-HACC NPsNDV F gene plasmid DNA
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