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Engineered pH-Responsive Mesoporous Carbon Nanoparticles for Drug Delivery
journal contribution
posted on 2020-03-17, 17:06 authored by Miguel Gisbert-Garzarán, Julia C. Berkmann, Dimitra Giasafaki, Daniel Lozano, Konstantinos Spyrou, Miguel Manzano, Theodore Steriotis, Georg N. Duda, Katharina Schmidt-Bleek, Georgia Charalambopoulou, María Vallet-RegíIn
this work, two types of mesoporous carbon particles with different
morphology, size, and pore structure have been functionalized with
a self-immolative polymer sensitive to changes in pH and tested as
drug nanocarriers. It is shown that their textural properties allow
significantly higher loading capacity compared to typical mesoporous
silica nanoparticles. In vial release experiments of a model Ru dye
at pH 7.4 and 5 confirm the pH-responsiveness of the hybrid systems,
showing that only small amounts of the cargo are released at physiological
pH, whereas at slightly acidic pH (e.g., that of lysosomes), self-immolation
takes place and a significant amount of the cargo is released. Cytotoxicity
studies using human osteosarcoma cells show that the hybrid nanocarriers
are not cytotoxic by themselves but induce significant cell growth
inhibition when loaded with a chemotherapeutic drug such as doxorubicin.
In preparation of an in vivo application, in vial responsiveness of
the hybrid system to short-term pH-triggering is confirmed. The consecutive
in vivo study shows no substantial cargo release over a period of
96 h under physiological pH conditions. Short-term exposure to acidic
pH releases an experimental fluorescent cargo during and continuously
after the triggering period over 72 h.
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Cytotoxicity studiesvivo studypH 7.4cargo release96 hShort-term exposurevivo applicationtextural propertiesmodel Ru dyeself-immolative polymerDrug Deliverycell growth inhibition72 hchemotherapeutic drugvial responsivenessloading capacitypore structureosteosarcoma cells showmesoporous silica nanoparticlesdrug nanocarriersacidic pH releasesmesoporous carbon particlesEngineered pH-Responsive Mesoporous Carbon Nanoparticlesvial release experimentspH conditionsacidic pH
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