Encapsulation of Microperoxidase‑8 in MIL-101(Cr)‑X
Nanoparticles: Influence of Metal–Organic Framework Functionalization
on Enzymatic Immobilization and Catalytic Activity
posted on 2020-03-19, 21:44authored byEffrosyni Gkaniatsou, Rémy Ricoux, Kalani Kariyawasam, Ingrid Stenger, Benhui Fan, Narjès Ayoub, Samanta Salas, Gilles Patriarche, Christian Serre, Jean-Pierre Mahy, Nathalie Steunou, Clémence Sicard
Microperoxidase
8 (MP8) was immobilized within MIL-101(Cr) bearing
terephthalate linkers with functionalized groups (-NH2 and
-SO3H). A synthesis protocol for MIL-101(Cr)-SO3H that avoids the use of toxic Cr(VI) and HF was developed. The electrostatic
interactions between the MP8 molecules and the MOF matrices were found
to be crucial for a successful immobilization. Raman spectroscopy
revealed the dispersion of the immobilized MP8 molecules in MIL-101(Cr)-X
matrices as monomers without aggregation. The presence of functional
groups resulted in higher amounts of immobilized MP8 in comparison
to the bare MIL-101(Cr). The catalytic activity of MP8@MIL-101(Cr)-NH2 per material mass was higher than that for MP8@MIL-101(Cr).
The presence of free amino groups can thus improve the immobilization
efficiency, leading to a higher amount of catalytically active species
and improving the subsequent catalytic activity of the heterogeneous
biocatalysts. MP8@MIL(Cr)-X also successfully catalyzed the selective
oxidation of thioanisole derivatives into sulfoxides.