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Emergence of DNA-Encapsulating Liposomes from a DNA–Lipid Blend Film
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posted on 2014-09-11, 00:00 authored by Shunsuke F. Shimobayashi, Masatoshi IchikawaSpontaneous
generation of DNA-enclosing liposomes from a DNA–lipid
blend film is investigated. The special properties of the lipid vesicles,
namely, micrometer size, unilamellarity, and dense polymer encapsulation
acquired by the dehydration–rehydration process, are physicochemically
revealed. We found that the formation of giant unilamellar vesicles
encapsulating DNAs are governed by micropatterns of the films, such
as dots and network patterns. From the results, we proposed a plausible
physical mechanism for the dehydration–rehydration process,
making it possible to optimize the encapsulation of any agent.