jf8b02431_si_001.pdf (1.31 MB)
Effects of Transgenic Bt Rice Containing the Cry1Ab Protein on the Gastrointestinal Health of Highly Inbred Wuzhishan Pigs after Two Generations of Feeding
journal contribution
posted on 2018-09-12, 00:00 authored by Qiang Liu, Weigang Yang, Mingjie Li, Yingzheng Wang, Hanxue Zhang, Yi Wu, Hui Gao, Ying Han, Xiangxia Bu, Shenming ZengTo
assess the safety of transgenic Bt rice, we evaluated the chronic
effects of Bt rice carrying the Cry1Ab protein (1.64 mg/kg) on the
gut microbiota and histopathology in highly inbred Wuzhishan pigs
(WZSP) in a two-generation feeding study. The WZSP of the f0 (n = 26) and f1 (n = 27) generations were
divided into GM and Non-GM groups and fed for 360 and 420 days with
Bt and isogenic parent-line rice, respectively. The high-throughput
16S rRNA gene sequencing results revealed that the relative abundance
of the family Enterobacteriaceae and genus Escherichia were significantly different between f1F and
f1Z. The level of the genera CF231 and SMB53 in the f0Z group was
higher than that in the f0F group. In addition, no abnormal histopathology
changes in the main gastrointestinal tissues were found after feeding
with Bt rice. In conclusion, Bt rice consumption has no adverse effects
on the gut microbiota or organ function of WZSP.