es0489083_si_001.pdf (58.01 kB)
Effect of Lot Variability on Ultraviolet Radiation Inactivation Kinetics of Cryptosporidium parvum Oocysts
journal contribution
posted on 2005-06-01, 00:00 authored by Mano Sivaganesan, Siva SivaganesanNumerous studies have demonstrated the efficiency of
ultraviolet (UV) radiation for the inactivation of oocysts of
Cryptosporidium parvum. In these studies inactivation is
measured as reduction in oocysts. A primary goal is to estimate
the UV radiation required to achieve a high degree of
inactivation. Different lots of Cryptosporidium parvum oocysts
are used in these studies, and the inactivation rate may
vary depending on the lot of oocysts used. The goal of this
paper is to account for the error in estimating the
amount of inactivation after exposure to UV radiation, and
for the effect of lot variability in determining the required
UV radiation. A Bayesian approach is used to simultaneously
model the logistic dose−response model and the UV
inactivation kinetic model. The oocysts lot variability is
incorporated using a hierarchical Bayesian model. Posterior
distributions using Markov Chain Monte Carlo method is
used to obtain estimates and Bayesian credible interval for
the required UV radiation to achieve a given inactivation
level of Cryptosporidium parvum oocysts.