nn9b08166_si_001.pdf (1.46 MB)
Dynamic Programming of a DNA Walker Controlled by Protons
journal contribution
posted on 2020-03-17, 20:03 authored by Dongbao Yao, Sanchita Bhadra, Erhu Xiong, Haojun Liang, Andrew D. Ellington, Cheulhee JungWe
have now constructed a four-legged DNA walker based on toehold
exchange reactions whose movement is controlled by alternating pH
changes. A well-characterized, pH-responsive CG-C+ triplex
DNA was embedded into a tetrameric catalytic hairpin assembly (CHA)
walker. The proton-controlled walker could autonomously move on otherwise
unprogrammed microparticles surface, and the walking rate and steps
of walking were efficiently controlled by pH. The starting and stopping
of the walker, and its association and dissociation from the microparticles,
could also be dynamically controlled by pH. The simple, programmable,
and robust nature of this proton-controlled walker now provides the
impetus for the development of a wide variety of more practical nanomachines.