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Dual-Mode Ultrasensitive Detection of Nucleic Acids via an Aqueous “Seesaw” Strategy by Combining Aggregation-Induced Emission and Plasmonic Colorimetry
journal contribution
posted on 2018-11-16, 00:00 authored by Jianlei Shen, Yiru Zhang, Rong Hu, Ryan T. K. Kwok, Zhiming Wang, Anjun Qin, Ben Zhong TangDevelopment of simple,
robust, and reliable nucleic acid detection
strategy holds tremendous promise for cancer or pathogen diagnostics.
However, in traditional fluorescent assays, tedious chemical labeling
and enzyme-based signal amplification are usually required. Developing
label-free, enzyme-free nucleic acid detection strategies is of great
significance. In this paper, through combining the hybridization chain
reaction (HCR) technique with aggregation-induced emission (AIE)-based
fluorometric assay and a gold-nanoparticle-based colorimetric assay,
we developed a “seesaw” strategy for label-free, enzyme-free
visual detection of nucleic acids. Two sets of signals (solution tonality
and fluorescence intensity changes) were generated for one test. The
limit of detection of single-strand DNA can be as low as 37.2 fM.
The proposed seesaw strategy that integrates fluorometric and colorimetric
assay holds great potential for the point-of-care testing of nucleic
acids.
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seesaw strategysingle-strand DNAgold-nanoparticle-based colorimetric assaylabel-freeAIEsolution tonalityacid detection strategiesacid detection strategyenzyme-freeAggregation-Induced EmissionDual-Mode Ultrasensitive DetectionHCRcolorimetric assayfluorescence intensity changeshybridization chain reaction37.2 fMpoint-of-care testingaggregation-induced emissionenzyme-based signal amplificationfluorometricNucleic Acidspathogen diagnosticsPlasmonic Colorimetry Development
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