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Dose-Dependent Effects of Triclocarban Exposure on Lipid Homeostasis in Rats
journal contribution
posted on 2019-10-11, 20:14 authored by Manyuan Dong, Xiaoyi Xu, Qingxia Huang, Hehua Lei, Guangyong Xu, Jianfeng Ma, Emmanuel Hatzakis, Xian Wang, Limin ZhangEnvironmental exposure to triclocarban
(TCC), a common antibacterial
agent widely used in thousands of personal care products, poses a
potential risk for human health. Previous in vitro studies about biological
effects of TCC have yielded a variety of inconsistent results and
apparently not been verified in vivo. In the current study, dose-dependent
effects of TCC exposure on lipid homeostasis in rats were investigated
using a combination of untargeted 1H NMR metabolomics,
targeted metabolite profiling (LC/GC–MS), histopathological
assessments, and biological assays. Our results revealed that TCC
dose-dependently activated aryl hydrocarbon receptor (AHR) and its
transcriptional targets such as Cyp1a1 and Cyp1b1 in the liver of rats, suggesting that TCC may be
a potent AHR agonist. Although TCC exhibited dose-dependent toxicity,
oral exposure with relatively low dose TCC caused more significant
hepatic lipogenesis of rats than relatively high and moderate doses
of TCC. It was mainly manifested by histopathological observations
and promotion of de novo fatty acid, phospholipid, and ceramide biosynthesis
and gut microbiota fermentation. Our findings provide new insights
into health effects of TCC exposure with different dosages in vivo,
especially on the induction and progression of nonalcoholic fatty
liver disease, and further our understanding in the pathogenesis of
metabolic diseases induced by environmental pollutants.