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Disruption of ζ‑Carotene Desaturase Protein ALE1 Leads to Chloroplast Developmental Defects and Seedling Lethality
journal contribution
posted on 2019-10-09, 17:03 authored by Yunxia Fang, Linlin Hou, Xiaoqin Zhang, Jiangjie Pan, Deyong Ren, Dali Zeng, Longbiao Guo, Qian Qian, Jiang Hu, Dawei Xueζ-carotene desaturase (ZDS)
is a key enzyme in carotenoid
biosynthesis and plays an important role in plant photosynthesis.
We characterized an albino leaf-color mutant obtained from ethyl methanesulfonate
treatment: albino and seedling lethality 1 (ale1).
The material contains a chloroplast thylakoid defect where photosynthetic
pigments declined and reactive oxygen species accumulated resulting
in ale1 death within 3 weeks. Positional cloning
and sequencing revealed that there was a single base substitution
in ALE1, which encoded a ZDS involved in carotenoid
biosynthesis. RNAi and complementation tests confirmed the identity
of ALE1. Subcellular localization showed that the
ALE1 protein is localized in the chloroplast. Expression analysis
indicated that the genes involved in chlorophyll and carotenoid biosynthesis
were downregulated. We conclude that ALE1 plays an
important role in chloroplast and plant growth in rice.