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Discrimination of Four Marine Biofilm-Forming Bacteria by LC–MS Metabolomics and Influence of Culture Parameters
journal contribution
posted on 2017-03-31, 00:00 authored by Laurie Favre, Annick Ortalo-Magné, Stéphane Greff, Thierry Pérez, Olivier P. Thomas, Jean-Charles Martin, Gérald CulioliMost
marine bacteria can form biofilms, and they are the main components
of biofilms observed on marine surfaces. Biofilms constitute a widespread
life strategy, as growing in such structures offers many important
biological benefits. The molecular compounds expressed in biofilms
and, more generally, the metabolomes of marine bacteria remain poorly
studied. In this context, a nontargeted LC–MS metabolomics
approach of marine biofilm-forming bacterial strains was developed.
Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model
organisms. The main objective was to search for some strain-specific
bacterial metabolites and to determine how culture parameters (culture
medium, growth phase, and mode of culture) may affect the cellular
metabolism of each strain and thus the global interstrain metabolic
discrimination. LC–MS profiling and statistical partial least-squares
discriminant analyses showed that the four strains could be differentiated
at the species level whatever the medium, the growth phase, or the
mode of culture (planktonic vs biofilm). A MS/MS molecular network
was subsequently built and allowed the identification of putative
bacterial biomarkers. TC8 was discriminated by a series of ornithine
lipids, while the P. mediterranea strains produced
hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine
derivatives, such as putrescine amides.