jm9b00931_si_001.pdf (654.35 kB)
Discovery of N‑Substituted 3‑Amino-4-(3-boronopropyl)pyrrolidine-3-carboxylic Acids as Highly Potent Third-Generation Inhibitors of Human Arginase I and II
journal contribution
posted on 2019-08-23, 19:03 authored by Michael C. Van Zandt, G. Erik Jagdmann, Darren L. Whitehouse, Minkoo Ji, Jennifer Savoy, Olga Potapova, Alexandra Cousido-Siah, Andre Mitschler, Eduardo I. Howard, Anna Marie Pyle, Alberto D. PodjarnyRecent efforts to
identify new highly potent arginase inhibitors
have resulted in the discovery of a novel family of (3R,4S)-3-amino-4-(3-boronopropyl)pyrrolidine-3-carboxylic
acid analogues with up to a 1000-fold increase in potency relative
to the current standards, 2-amino-6-boronohexanoic acid (ABH) and N-hydroxy-nor-l-arginine (nor-NOHA). The lead candidate,
with an N-2-amino-3-phenylpropyl substituent (NED-3238),
example 43, inhibits arginase I and II with IC50 values of 1.3 and 8.1 nM, respectively. Herein, we report the design,
synthesis, and structure–activity relationships for this novel
series of inhibitors, along with X-ray crystallographic data for selected
examples bound to human arginase II.