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Discovery of Neolignan Glycosides with Acetylcolinesterase Inhibitory Activity from Huangjinya Green Tea Guided by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry Data and Global Natural Product Social Molecular Networking
journal contribution
posted on 2019-10-18, 18:36 authored by Hao-Yue Wu, Jia-Ping Ke, Wei Wang, Ya-Shuai Kong, Peng Zhang, Tie-Jun Ling, Guan-Hu BaoGlobal Natural Product Social feature-based
networking was applied
to follow the phytochemicals, including nine flavonoid glycosides,
six catechins, and three flavonols in Huangjinya green tea. Further,
a new 8-O-4′-type neolignan glycoside, camellignanoside
A (1), and 15 known compounds (2–16) were isolated through a variety of column chromatographies,
and the structure was elucidated extensively by ultra performance
liquid chromatography–quadrupole–time-of-flight–tandem
mass spectrometry, 1H and 13C nuclear magnetic
resonance, heteronuclear single-quantum correlation, heteronuclear
multiple-bond correlation, 1H–1H correlation
spectroscopy, rotating frame nuclear Overhauser effect spectroscopy,
and Nuclear Overhauser effect spectroscopy, and circular dichroism
spectroscopies. Compounds 1 and 2 showed
acetylcolinesterase inhibition activity, with IC50 = 0.75
and 0.18 μM, respectively.
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Overhauser effect spectroscopyflavonoid glycosides13 Cacetylcolinesterase inhibition activity0.18 μ MHuangjinya Green Tea GuidedAcetylcolinesterase Inhibitory Activityheteronuclear multiple-bond correlationCompounds 1column chromatographiesNeolignan Glycosidesheteronuclear single-quantum correlation1 Hdichroism spectroscopiesIC 50Global Natural Product Social Molecular Networking Global Natural Product Social feature-based networkingNuclear Overhauser effect spectroscopy
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