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Direct Three-Dimensional Characterization and Multiscale Visualization of Wheat Straw Deconstruction by White Rot Fungus
journal contribution
posted on 2014-08-19, 00:00 authored by Li Liu, Chen Qian, Lei Jiang, Han-Qing YuMicrobial
degradation of lignocellulose for resource and energy
recovery has received increasing interest. Despite its obvious importance,
the mechanism behind the biodegradation, especially the changes of
morphological structure and surface characteristics, has not been
fully understood. Here, we used three-dimensional (3D) characterization
and multiscale visualization methods, in combination with chemical
compositional analyses, to elucidate the degradation process of wheat
straw by a white rot fungus, Phanerochaete chrysosporium. It was found that the fungal attack initiated from stomata. Lignin
of the straw decayed in both size and quantity, and heterogeneity
in the biodegradation was observed. After treatment with the fungus,
the straw surface turned from hydrophobic to hydrophilic, and the
adhesion of the straw surface increased in the fungal degradation.
The morphology of the straw outer layer became heterogeneous and loose
with the formation of many holes with various sizes. The wasp-tunnels-like
structure of the collenchyma and parenchyma of the straw as well as
the fungal hyphae interspersed inside the straw structure were clearly
visualized in the 3D reconstruction structure. This work offers a
new insight into the mechanism of lignocellulose biodegradation and
demonstrates that multiscale visualization methods could be a useful
tool to explore such complex processes.