se8b00736_si_001.pdf (190.16 kB)
Direct Observation of Oxidation Reaction via Closed Bipolar Electrode-Anodic Electrochemiluminescence Protocol: Structural Property and Sensing Applications
journal contribution
posted on 2018-10-16, 00:00 authored by Jia-Dong Zhang, Lei Lu, Xiu-Fang Zhu, Li-Jing Zhang, Shan Yun, Chuan-Song Duanmu, Lei HeIn this work, we developed an innovative
closed bipolar electrode
(BPE)-electrochemiluminescence (ECL) sensing strategy with generality
for target detection. Based on charge balance and 100% current efficiency
between the closed BPE poles and the driving electrodes, one of the
driving electrodes in one cell of the closed BPE system was employed
as ECL sensing surface to reflect the target on the BPE pole in the
opposite cell. Compared with traditional BPE-ECL sensing method, which
in general adopted the anodic ECL reagents such as Ru(bpy)32+ and its coreactant on one pole (anode) to reflect the
target (occurring reduction reaction) on the other pole (cathode),
the difference was that the targets occurring oxidation reaction could
be detected by the anodic ECL reagents based on this strategy. To
verify the feasibility of this strategy, the detection principle was
stated first, and Fe(CN)64– as model
target at anodic BPE pole were detected by anodic ECL reagents (Ru(bpy)32+ and TprA) on the driving electrode first. The
ECL signals showed good performance for target detection. By changing
the size and the material of the BPE pole where the targets were located,
the detection of l-ascorbic acid (AA), uric acid (UA), and
dopamine (DA) as other model targets with higher detection limit were
accomplished. Visual and high-throughput detection of AA, UA, and
DA were also successfully realized by an array of the closed BPE system.
This closed BPE (array) system is an effective supplement of traditional
BPE-ECL sensing and could greatly expand the scope of the detection
target.