se8b01498_si_001.pdf (404.65 kB)
Direct Monitoring of Cancer-Associated mRNAs in Living Cells to Evaluate the Therapeutic RNAi Efficiency Using Fluorescent Nanosensor
journal contribution
posted on 2019-04-19, 00:00 authored by Seong
Min Ahn, Seounghun Kang, Dal-Hee MinCancer-associated
mRNA (mRNA) is an important biomarker for early
diagnosis, prognosis, and prediction of treatment responses. Despite
recent developments in fluorescence live cell imaging, reliable detection
and quantification of mRNA in living cells still remain challenging
due to a complicated intracellular environment. Herein, we present
a fluorescent nanosensor for live-cell monitoring of cancer-related
mRNAs involved in the canonical Wnt/β-catenin signaling pathway.
The nanosensor enables rapid and accurate assessment of gene downregulation
efficiency in a dose- and time-dependent manner by measuring quantitative
fluorescence signal corresponding to β-catenin or its target
mRNA levels in living cells. It is expected that the fluorescent nanosensor
will be applicable to high-throughput screening for the efficient
drug discovery and insightful understanding of the molecular mechanisms
of potential drug candidate for cancer treatment.
History
Usage metrics
Categories
Keywords
cell imagingdrug discoveryfluorescence signalTherapeutic RNAi Efficiencytime-dependent mannerlive-cell monitoringnanosensorCancer-Associated mRNAsgene downregulation efficiencyintracellular environmentLiving Cellscancer treatmentFluorescent Nanosensor Cancer-associated mRNADirect Monitoringdrug candidateβ- catenintarget mRNA levelstreatment responseshigh-throughput screening
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC