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Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses
journal contribution
posted on 2020-04-01, 13:03 authored by Natália Ellen Castilho de Almeida, Franciele Grego Esteves, José Roberto
Aparecido dos Santos-Pinto, Carla Peres de Paula, Anderson Ferreira da Cunha, Iran Malavazi, Mario Sergio Palma, Edson Rodrigues-FilhoCeliac disease (CD) is a chronic
illness characterized by an inflammatory
process triggered by gluten protein intake. Recent evidence has suggested
that the lower relative abundance of bifidobacteria in the intestinal
lumen may be associated with CD. Herein, we assessed the effect of
the Bifidobacterium species Bifidobacterium
bifidum, Bifidobacterium longum, Bembidion breve, Bifidobacterium animalis alone, and also a Bifidobacterium consortium on the digestion of intact gluten
proteins (gliadins and glutenins) and the associated immunomodulatory
responses elicited by the resulting peptides. The cytotoxicity and
proinflammatory responses were evaluated through the activation of
NF-kB p65 and the expression of cytokines TNF-α and IL-1β
in Caco-2 cell cultures exposed to gluten-derived peptides. The peptides
induced a clear reduction in cytotoxic responses and proinflammatory
marker levels compared to the gluten fragments generated during noninoculated
gastrointestinal digestion. These results highlight the possible use
of probiotics based on bifidobacteria as a prospective treatment for
CD.
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Bifidobacterium Speciesgluten fragmentsBifidobacterium species Bifidobacterium bifidumgluten-derived peptidesIntact Gluten Proteinsimmunomodulatory responsesNF-kB p 65gluten protein intakeproinflammatory responsesproinflammatory marker levelsBifidobacterium animalisBembidion breveBifidobacterium longumCaco -2 cell culturescytotoxic responsesIL -1βBifidobacterium consortiumcytokines TNF -αProinflammatory Responses Celiac diseasegluten proteinsRecent evidence
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