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Differential Proteomic Analysis of Cancer Stem Cell Properties in Hepatocellular Carcinomas by Isobaric Tag Labeling and Mass Spectrometry
journal contribution
posted on 2013-08-02, 00:00 authored by Ching-Huai Ko, Chieh-Fang Cheng, Chin-Pen Lai, Te-Hui Tzu, Chih-Wei Chiu, Mei-Wei Lin, Si-Yuan Wu, Chung-Yuan Sun, Hsiang-Wen Tseng, Chun-Chung Wang, Zong-Keng Kuo, Ling-Mei Wang, Sung-Fang ChenMalignant
tumors are relatively resistant to treatment due to their
heterogeneous nature, drug resistance, and tendency for metastasis.
Recent studies suggest that a subpopulation of cancer cells is responsible
for the malignant outcomes. These cells are considered as cancer stem
cells (CSC). Although a number of molecules have been identified in
different cancer cells as markers for cancer stem cells, no promising
markers are currently available for hepatocellular carcinoma cells.
In this study, two clones of Hep3B cell lines were functionally characterized
as control or CSC-like cells, based on properties including spheroid
formation, drug resistance, and tumor initiation. Furthermore, their
protein expression profiles were investigated by isobaric tags for
relative and absolute quantitation (iTRAQ), and a total of 1,127 proteins
were identified and quantified from the combined fractions; 50 proteins
exhibited at least 2-fold differences between these two clones. These
50 proteins were analyzed by GeneGo and were found to be associated
with liver neoplasms, hepatocellular carcinoma (HCC), and liver diseases.
They were also components of metabolic pathways, immune responses,
and cytoskeleton remodeling. Among these proteins, the expressions
of S100P, S100A14, and vimentin were verified in several HCC cell
lines, and their expressions were correlated with tumorigenicity in
HCC cell lines. The functional significance of vimentin and S100A14 were also investigated
and verified.